Quantitative real-time PCR assay for QPX (Thraustochytriidae), a parasite of the hard clam (Mercenaria mercenaria).
نویسندگان
چکیده
We developed a real-time quantitative PCR (qPCR) assay targeting the rRNA internal transcribed spacer region of the hard clam pathogen QPX. The qPCR assay was more sensitive than was histology in detecting clams with light QPX infections. QPX was detected in 4 of 43 sediment samples but in none of 40 seawater samples.
منابع مشابه
Seasonality of QPX disease in the Raritan Bay (NY) wild hard clam (Mercenaria mercenaria) population
Quahog Parasite Unknown (QPX) is a potentially lethal pathogen of the hard clam Mercenaria mercenaria along the northeastern coast of the United States. In the Raritan Bay wild clam population, QPX prevalence and infection intensity at three sites were examined by both histology and quantitative PCR in 2006. At two of three sites, QPX infection showed a seasonal pattern, with prevalence and wei...
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Quahog Parasite Unknown (QPX) is a fatal protistan parasite that causes severe losses in the hard clam (Mercenaria mercenaria) fisheries along the northeastern coast of the US. Field and laboratory studies of QPX disease have demonstrated a major role for water temperature and M. mercenaria genetic origin in disease development. Infections are more likely to occur at cold temperatures, with cla...
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The ability of pathogens to neutralize host defence mechanisms represents a fundamental requisite in the successful establishment of an infection. Host-pathogen interactions between quahog parasite unknown (QPX) and its hard clam host are poorly understood. Our prior in vivo investigations have shown that different QPX isolates display varying levels of pathogenicity toward clams. Similarly, fi...
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Quahog parasite unknown (QPX) is a fatal protistan parasite affecting cultured and wild hard clams Mercenaria mercenaria along the northeastern coasts of the USA and maritime Canada. Field investigations and laboratory transmission studies revealed some variations in the susceptibility of different hard clam stocks to QPX infection. In this study, we used in vitro QPX cultures to investigate th...
متن کاملEffects of salinity on hard clam (Mercenaria mercenaria) defense parameters and QPX disease dynamics.
QPX (Quahog Parasite Unknown) is a protistan parasite affecting hard clams (Mercenaria mercenaria) along the Northeast coast of the United States. The fact that QPX disease epizootics are usually observed in field sites with high salinities led to the general assumption that salinity represents an important factor for disease distribution. This study was designed to investigate the effect of sa...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 75 14 شماره
صفحات -
تاریخ انتشار 2009